Journal: Journal of Orthopaedic Surgery and Research
Article Title: Serum DLX6-AS1 and miR-141-3p as early diagnostic biomarkers and regulators of delayed fracture healing (DFH)
doi: 10.1186/s13018-025-06478-5
Figure Lengend Snippet: VEGFA is a binding target of miR-141-3p. A Predicted binding sequence. B Dual-luciferase assay validation. C VEGFA expression levels in NFH (n = 96) vs DFH (n = 96) patients. D , E Correlation analyses between VEGFA and miR-141-3p or DLX6-AS1 (n = 96). Data are presented as mean ± SD. Statistical analysis was performed using Student’s t-test or one-way ANOVA. hBMSCs, Human bone marrow mesenchymal stem cells. NFH, normal fracture healing; DFH, delayed fracture healing. ** P < 0.01, *** P < 0.001
Article Snippet: Human bone marrow mesenchymal stem cells (hBMSCs) were obtained from ATCC (USA).
Techniques: Binding Assay, Sequencing, Luciferase, Biomarker Discovery, Expressing